Kanako Miyamae wanted to discover true love when she transferred to a new all-girls school. Instead, she discovered the lovely Mariya Shidou, a fascinating classmate Kanako met on her first day. Mariya, on the other hand, has a flaw: she is a cross-dressing guy! Kanako's life has been a succession of tragedies since uncovering Mariya's secret and being stuck with him as her roommate. All she wants is to discover the girl of her dreams, which is difficult with the interfering of the wicked Mariya and his sassy maid Matsurika Shinouji. MariaHolic Alive follows Kanako's journey as she cope with being Mariya's roommate and tries to discover true love while surviving high school.